Inactivation of viral and prion pathogens by γ‐irradiation under conditions that maintain the integrity of human albumin
- 24 January 2003
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 84 (1), 36-44
- https://doi.org/10.1046/j.1423-0410.2003.00256.x
Abstract
Background and Objectives The administration of therapeutic plasma protein concentrates has been associated with the real risk of transmitting viral diseases and the theoretical risks of prion transmission. Our objective was to determine if γ‐irradiation can inactivate viral or prion infectivity without damaging a protein biotherapeutically. Materials and Methods Human albumin 25% solution, spiked with four model viruses (including porcine parvovirus) or with brain homogenate from scrapie‐infected hamsters, was γ‐irradiated at constant low‐dose rates and assayed for viral and prion infectivity or for albumin integrity. Results At a radiation dose of 50 kGy, viruses were inactivated by ≥ 3·2 to ≥ 6·4 log10 and scrapie by an estimated 1·5 log10, whereas albumin was only moderately aggregated and fragmented. Conclusions γ‐Irradiation can preferentially inactivate viral and prion pathogens without excessive damage to albumin structure.Keywords
This publication has 28 references indexed in Scilit:
- The Sterways Process: a New Approach to Inactivating Viruses Using Gamma RadiationBiologicals, 1998
- Inactivation of human immunodeficiency virus by gamma radiation and its effect on plasma and coagulation factorsTransfusion, 1991
- Effect of Gamma Irradiation on the Human Immunodeficiency Virus and Human Coagulation ProteinsVox Sanguinis, 1989
- Estimation of scrapie nucleic acid MW from standard curves for virus sensitivity to ionizing radiationNature, 1986
- Inactivation of viruses in labile blood derivatives. II. Physical methodsTransfusion, 1985
- Measurement of the scrapie agent using an incubation time interval assayAnnals of Neurology, 1982
- The Scrapie Agent: Evidence Against its Dependence for Replication on Intrinsic Nucleic AcidJournal of General Virology, 1978
- Radiosensitive Molecular Weight of Single-Stranded Virus Nucleic AcidsNature, 1963
- Radiosensitive Molecular Weight of Tobacco Mosaic Virus Nucleic AcidNature, 1957
- Comparison of Some Direct and Indirect Effects of Ionizing Radiations in ProteinNature, 1956