Determination of uromodulin in human urine: influence of storage and processing
Open Access
- 3 October 2013
- journal article
- research article
- Published by Oxford University Press (OUP) in Nephrology Dialysis Transplantation
- Vol. 29 (1), 136-145
- https://doi.org/10.1093/ndt/gft345
Abstract
Uromodulin (Tamm–Horsfall protein) is the most abundant protein excreted in the urine under physiological conditions. It is exclusively produced in the kidney and secreted into the urine via proteolytic cleavage. The involvement of UMOD, the gene that encodes uromodulin, in rare autosomal dominant diseases, and its robust genome-wide association with the risk of chronic kidney disease suggest that the level of uromodulin in urine could represent a critical biomarker for kidney function. The structure of uromodulin is complex, with multiple disulfide bonds and typical domains of extracellular proteins. Thus far, the conditions influencing stability and measurement of uromodulin in human urine have not been systematically investigated, giving inconsistent results. In this study, we used a robust, in-house ELISA to characterize the conditions of sampling and storage necessary to provide a faithful dosage of uromodulin in the urine. The levels of uromodulin in human urine were significantly affected by centrifugation and vortexing, as well as by the conditions and duration of storage. These results validate a simple, low-cost ELISA and document the optimal conditions of processing and storage for measuring uromodulin in human urine.This publication has 28 references indexed in Scilit:
- Phenotype and Outcome in Hereditary Tubulointerstitial Nephritis Secondary to UMOD MutationsClinical Journal of the American Society of Nephrology, 2011
- The rediscovery of uromodulin (Tamm–Horsfall protein): from tubulointerstitial nephropathy to chronic kidney diseaseKidney International, 2011
- Activation of the Bumetanide-sensitive Na+,K+,2Cl− Cotransporter (NKCC2) Is Facilitated by Tamm-Horsfall Protein in a Chloride-sensitive MannerJournal of Biological Chemistry, 2011
- Tamm-Horsfall Glycoprotein Interacts with Renal Outer Medullary Potassium Channel ROMK2 and Regulates Its FunctionOnline Journal of Public Health Informatics, 2011
- Urinary Biomarkers in the Clinical Prognosis and Early Detection of Acute Kidney InjuryClinical Journal of the American Society of Nephrology, 2010
- Mutations in the uromodulin gene decrease urinary excretion of Tamm-Horsfall proteinKidney International, 2004
- A Cluster of Mutations in the UMOD Gene Causes Familial Juvenile Hyperuricemic Nephropathy with Abnormal Expression of UromodulinJournal of the American Society of Nephrology, 2003
- A panoramic view of gene expression in the human kidneyProceedings of the National Academy of Sciences of the United States of America, 2003
- Tamm-Horsfall glycoprotein: biology and clinical relevanceAmerican Journal of Kidney Diseases, 2003
- Uromucoid (Tamm-Horsfall glycoprotein) forms different polymeric arrangements on a filter surface under different physicochemical conditionsClinica Chimica Acta; International Journal of Clinical Chemistry, 1987