Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic

Abstract

A rather fast and complicated progression of an infection caused by some strains ofStaphylococcus aureuscould be associated with the expression and co-action of virulence factor complexes in these strains. This study screened the antibiotic susceptibility and prevalence of virulence markers in isolates of meticillin-resistantS. aureus(MRSA) obtained from patients hospitalized at the University Hospital in Olomouc, Czech Republic. A total of 100 isolates was screened for 13 genes encoding extracellular virulence determinants (tst,pvl,eta,etb,sea,seb,sec,sed,see,seg,seh,seiandsej) and for their distribution in sample types. Eighty-nine isolates were positive for at least one of the genes. Genes foretb,pvl,seeandsehwere not detected in any of the MRSA isolates. No statistically significant differences in the occurrence of the determinants studied among sample types were found.