Short-term very low-calorie diet in obese females improves the haemostatic balance through the reduction of leptin levels, PAI-1 concentrations and a diminished release of platelet and leukocyte-derived microparticles
- 8 March 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in International Journal of Obesity
- Vol. 35 (12), 1479-1486
- https://doi.org/10.1038/ijo.2011.19
Abstract
In obesity, metabolic stress and inflammation in injured tissues could favour enhanced shedding of procoagulant microparticles (MPs). At sites of endothelium injury, the swift recruitment of procoagulant leukocyte-derived MPs enables the initiation of blood coagulation and thrombus growth. In obese females, we sought to evaluate the impact of a very low-calorie diet (VLCD) on procoagulant MP levels, fibrinolytic status, inflammation and endothelium damage. Circulating biomarkers of vascular damage, fibrinolytic status, platelet activation and inflammation were measured before, 30 and 90 days after starting a short-term VLCD. MPs were measured by flow cytometry and capture assays. Their procoagulant potential was quantified using functional prothrombinase assays and their cellular origin were determined using flow cytometry (endothelium, platelet, leukocyte, lymphocyte and erythrocyte-derived MP) or capture assays. A total of 24 obese females (39 ± 10 years) with a mean body mass index of 35 ± 4 kg m(-2) were prospectively enroled. Procoagulant leukocyte-derived MPs were associated with the waist circumference at baseline (r=0.534; P=0.010) and at 90 days follow-up (r=0.487; P=0.021). At 90 days, weight reduction (-9.8%) was associated with a lowering of blood pressure, improvement of metabolic parameters and a significant reduction of plasminogen activator inhibitor-1 (PAI-1) (-38%), procoagulant platelet-derived MPs (-43%), leukocyte-derived MPs (-28%) and leptin (-32%) levels. In obese females, a short-term VLCD results in an overall improvement of the haemostatic balance characterized by the reduction of PAI-levels, diminished release of platelet and leukocyte-derived MPs and a reduction in leptin levels, an adipocyte-derived cytokine.Keywords
This publication has 38 references indexed in Scilit:
- Leptin Resistance: A Possible Interface of Inflammation and Metabolism in Obesity-Related Cardiovascular DiseaseJournal of the American College of Cardiology, 2008
- Adipokines, Insulin Resistance, and Coronary Artery CalcificationJournal of the American College of Cardiology, 2008
- Level, distribution and correlates of platelet-derived microparticles in healthy individuals with special reference to the metabolic syndromeThrombosis and Haemostasis, 2008
- Increased levels of circulating microparticles in patients with severe aortic valve stenosisThrombosis and Haemostasis, 2008
- Procoagulant MicroparticlesArteriosclerosis, Thrombosis, and Vascular Biology, 2006
- Circulating Leukocyte-Derived Microparticles Predict Subclinical Atherosclerosis Burden in Asymptomatic SubjectsArteriosclerosis, Thrombosis, and Vascular Biology, 2006
- Hemostatic abnormalities associated with obesity and the metabolic syndromeJournal of Thrombosis and Haemostasis, 2005
- More on: measuring circulating cell-derived microparticlesJournal of Thrombosis and Haemostasis, 2005
- Postprandial elevation of tissue factor antigen in the blood of healthy adultsThrombosis and Haemostasis, 2005
- Leptin Induces Mitochondrial Superoxide Production and Monocyte Chemoattractant Protein-1 Expression in Aortic Endothelial Cells by Increasing Fatty Acid Oxidation via Protein Kinase APublished by Elsevier BV ,2001