Independent Regulation of MucD, an HtrA-Like Protease in Pseudomonas aeruginosa , and the Role of Its Proteolytic Motif in Alginate Gene Regulation
- 15 April 2006
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 188 (8), 3134-7
- https://doi.org/10.1128/jb.188.8.3134-3137.2006
Abstract
Expression of mucD , encoding a homologue of the HtrA(DegP) family of endoserine proteases, was investigated in Pseudomonas aeruginosa . Expressed from the algT-mucABCD operon, MucD was detected in mucoid (FRD1) and nonmucoid (PAO1) parental strains and also when polar insertions were placed upstream in algT or mucB . A transcriptional start site for a mucD promoter (P mucD ) was mapped within mucC . Expression of single-copy mucD217 , encoding MucD altered in the protease motif (S217A), was defective in temperature resistance and alginate gene regulation.Keywords
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