Bendazac Lysine Inhibition of Human Lens Epithelial Cell Adhesion to Polymethylmethacrylate Intraocular Lenses
- 23 April 2004
- journal article
- Published by S. Karger AG in Ophthalmic Research
- Vol. 36 (3), 145-150
- https://doi.org/10.1159/000077327
Abstract
The aim of the present work was to evaluate the effect of bendazac lysine on the human lens epithelial cell line HLE-B3 adhesion to polymethylmethacrylate (PMMA) intraocular lenses (IOLs). After adherence to IOLs, cells were incubated in the presence of the drug for 24 h. The number of cells contained in a 6-mm2 area was then counted with an inverted phase microscope and adherent cells were distinguished from detached floating cells by focusing through the medium. Results obtained show that bendazac is able to induce a linear dose-dependent inhibition of HLE-B3 adhesiveness to PMMA IOLs. In particular, treatment with bendazac 33, 100 and 300 µM resulted in a 15, 32 and 54% inhibition, respectively. Statistical analysis shows that this effect is significant at 100 µM (p < 0.05) and 300 µM (p < 0.01). The analysis of the effects of bendazac on the viability and on the proliferative capacity of HLE-B3 cells did not show any drug-related toxicity up to the concentration of 400 µM. The present study demonstrates that bendazac lysine is able to inhibit adhesion of lens epithelial cells to PMMA IOLs and suggests the potential beneficial use of this drug in preventing secondary cataract development.Keywords
This publication has 7 references indexed in Scilit:
- Posterior capsule opacification and lens epithelial cell layer formation: Hydroview hydrogel versus AcrySof acrylic intraocular lensesJournal of Cataract & Refractive Surgery, 2001
- Posterior capsular opacification with hydrogel, polymethylmethacrylate, and silicone intraocular lenses: two-year results of a randomized prospective trialAmerican Journal of Ophthalmology, 2000
- Steroid and anti-CD18 treatment have no effect on after-cataract formation following surgery in rabbitsCurrent Eye Research, 2000
- Inhibition of bacterial adherence to a high-water-content polymer by a water-soluble, nonsteroidal, anti-inflammatory drugJournal of Biomedical Materials Research, 1998
- A systematic overview of the incidence of posterior capsule opacificationOphthalmology, 1998
- Posterior capsule opacificationSurvey of Ophthalmology, 1992
- Comparison of in vitro cell cytotoxic assays for tumor necrosis factorJournal of Immunological Methods, 1984