Increased expression of lymphocyte‐derived cytokines in benign hyperplastic prostate tissue, identification of the producing cell types, and effect of differentially expressed cytokines on stromal cell proliferation
- 23 May 2002
- journal article
- research article
- Published by Wiley in The Prostate
- Vol. 52 (1), 43-58
- https://doi.org/10.1002/pros.10084
Abstract
BACKGROUND Benign prostatic hyperplasia (BPH) frequently exhibit infiltration of CD4 +/CD45RO + memory-T-lymphocytes. Expression and impact of lymphocyte-derived growth factors on prostatic stromal cell (PSC) growth were investigated. METHODS Lymphokine synthesis in normal prostate tissues (n = 3), BPH-tissues (n = 13), BPH-derived T-cells (n = 6), BPH-derived epithelial cells (BPH-EC) (n = 5), normal prostate-derived (n = 3) and BPH-derived stromal cell lines (BPH-SC) (n = 6), and prostate cancer (CaP) lines (n = 3) was analyzed by RT-PCR and Southern-blotting. The effect of interleukin (IL)-2, -4, -7, and interferon-γ (IFN-γ) on normal and BPH-SC growth was investigated by 3H-thymidine incorporation assays. RESULTS All BPH-tissues and, to a lesser degree, normal prostates, expressed significant amounts of IFN-γ mRNA. However, only BPH-tissues contained IL-2 and IL-4 mRNA (ratio: 10:13). BPH-T-cell lines were heterogenous in composition and expressed significant amounts of IFN-γ, IL-2, and IL-4 mRNA. Low level expression of these lymphokines was also observed in BPH-EC, CaP lines, and PSC lines. IL-2, -7 and IFN-γ stimulated the proliferation of BPH-PSC lines but not that of normal PSC, while IL-4 inhibited BPH-PSC growth. CONCLUSIONS Chronic inflammation may induce an increased growth pattern of fibromuscular tissue in BPH similar to that of wound healing. Prostate 52: 43–58, 2002.This publication has 48 references indexed in Scilit:
- Interleukin-4 and interferon-γ discordantly regulate collagen biosynthesis by functionally distinct lung fibroblast subsetsJournal of Cellular Physiology, 1996
- Cytoskeletal and Cytocontractile Protein Composition of Stromal Tissue in Normal, Hyperplastic, and Neoplastic Human ProstateAnnals of the New York Academy of Sciences, 1996
- The expanding universe of T-cell subsets: Th1, Th2 and moreImmunology Today, 1996
- Suppression of Hematopoietic Support Function Is Associated with Overexpression of lnterleukin-4 and Transforming Growth Factor-β1 in LP-BM5 Murine-Leukemia-Virus-lnfected Stromal Cell LinesActa Haematologica, 1996
- Defective functional response to membrane stimuli in lymphocytes from patients with benign prostatic hyperplasiaClinical and Experimental Immunology, 1995
- In vivo characterization of interleukin‐4 as a potential wound healing agentWound Repair and Regeneration, 1995
- Cytokine production and heterogeneity of primary stromal cells that support B lymphopoiesisEuropean Journal of Immunology, 1993
- Phenotypically diverse mouse thymic stromal cell lines which induce proliferation and differentiation of hematopoietic cellsEuropean Journal of Immunology, 1993
- Clonal growth of human prostatic epithelial cells is stimulated by fibroblastsThe Prostate, 1989
- Lymphokine regulation of inflammatory processes: Interleukin-4 stimulates fibroblast proliferationClinical Immunology and Immunopathology, 1988