Quantitative Study of Competitive Binding of Drugs to Protein by Microdialysis/High-Performance Liquid Chromatography
- 1 January 1998
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 70 (2), 373-377
- https://doi.org/10.1021/ac970564u
Abstract
A displacement equation describing competitive binding of drugs to protein in solution is derived and examined with four nonsteroidal anti-inflammatory drugs and human serum albumin as model drugs and protein, respectively. Microdialysis/high-performance liquid chromatography was adopted to determine simultaneously the unbound solute and displacing agent in drug−protein solutions. The method is able to locate the binding site and determine affinity constants even up to 107 L/mol accurately. A comparison of association constants determined by this method and from capacity factors on HSA−CSP is given.Keywords
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