The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
Open Access
- 1 January 1989
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 3 (1), 60-72
- https://doi.org/10.1101/gad.3.1.60
Abstract
Rapid degradation of c-fos proto-oncogene mRNA is crucial for transient c-fos gene expression. Experiments were performed to investigate the cellular mechanisms responsible for the extremely short half-life of human c-fos mRNA in growth-factor-stimulated fibroblasts. These experiments demonstrate the existence of two distinct cellular pathways for rapid c-fos mRNA degradation. Each of these pathways recognizes a different, functionally independent instability determinant within the c-fos transcript. One instability determinant, which is located within the c-fos 3'-untranslated region, is a 75-nucleotide AU-rich segment. Insertion of this element into beta-globin mRNA markedly reduces the half-life of that normally long-lived message. Nevertheless, specific deletion of the AU-rich element from c-fos mRNA has little effect on the transcript's cytoplasmic half-life due to the presence of the other c-fos instability determinant, which is located in the protein-coding segment of the c-fos message. Examination of mRNA decay in cells treated with transcription inhibitors indicates that one c-fos mRNA degradation pathway is dependent on RNA synthesis, whereas the other is not.This publication has 30 references indexed in Scilit:
- The c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genesCell, 1988
- At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cellsJournal of Molecular Biology, 1987
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Superinduction of c- fos by Nerve Growth Factor in the Presence of Peripherally Active BenzodiazepinesScience, 1985
- Regulation of messenger RNA stability in mouse erythroleukemia cellsJournal of Molecular Biology, 1985
- Induction of c-fos gene and protein by growth factors precedes activation of c-mycNature, 1984
- Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and proteinNature, 1984
- Expression of the c- fos Gene and of an fos -Related Gene Is Stimulated by Platelet-Derived Growth FactorScience, 1984
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Linker Tailing: Unphosphorylated Linker Oligonucleotides for Joining DNA TerminiDNA, 1984