High-Performance Liquid Chromatography with Photodiode Array Detection (HPLC−DAD)/HPLC−Mass Spectrometry (MS) Profiling of Anthocyanins from Andean Mashua Tubers (Tropaeolum tuberosumRuíz and Pavón) and Their Contribution to the Overall Antioxidant Activity
- 18 August 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (19), 7089-7097
- https://doi.org/10.1021/jf0614140
Abstract
Mashua (Tropaeolum tuberosum Ruíz and Pavón), an Andean tuber with high antioxidant activity, has sparked interest because of its traditional medicinal use. In this study, we evaluated the anthocyanin composition for three purple mashua genotypes and their contribution to the overall antioxidant activity of the tuber. Mashua anthocyanins, total phenolics, and 2,2‘-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant activity ranged from 45.5 to 131.9 mg of cyanidin 3-glucoside equivalents/100 g fresh weight (FW), 174.9 to 275.5 mg of gallic acid equivalents/100 g of FW, and 16.2 to 45.7 μmol of Trolox equivalents/g of FW, respectively. The high-performance liquid chromatography with photodiode array detection (HPLC−DAD) and HPLC−electrospray ionization tandem mass spectrometry (ESI/MS−MS) profiles revealed the presence of 11 different anthocyanins. The two major pigments (56.4−73.0% total area range at 520 nm) were identified as delphinidin 3-glucoside-5-acetylrhamnoside and delphinidin 3-sophoroside-5-acetylrhamnoside. Other pigments were delphinidin 3-glucoside-5-rhamnoside, delphinidin 3-sophoroside-5-rhamnoside, delphinidin 3-glucoside, cyanidin 3-sophoroside, and cyanidin 3-sophoroside-5-rhamnoside. Cyanidin 3-glucoside and cyanidin 3-rutinoside were only found in two genotypes, while pelargonidin 3-sophoroside and pelargonidin 3-sophoroside-5-rhamnoside were only found in the third one. Anthocyanins from mashua were the major contributors to the total ABTS values for only one of the three genotypes, suggesting that other phenolics present are playing a major role in the antioxidant power of mashua tubers. Results from this study provide important information for the Nutraceutical and Functional Food Market for the use of mashua anthocyanins not only as a source of natural colorants but also as a source of phytonutrients. Keywords: Mashua; anthocyanins; Tropaeolum tuberosum; antioxidant activity; HPLC−DAD; HPLC−ESI/MS−MS; total phenolicsKeywords
This publication has 16 references indexed in Scilit:
- Glucosinolates in isaño (Tropaeolum tuberosum) tubers: qualitative and quantitative content and changes after maturityJournal of the Science of Food and Agriculture, 2004
- LC‐MS identification of anthocyanins in boysenberry extract and anthocyanin metabolites in human urine following dosingJournal of the Science of Food and Agriculture, 2004
- Chromatographic Separation of Phenolic Compounds from Rapeseed by a Sephadex LH‐20 Column with Ethanol as the Mobile PhaseJournal of Liquid Chromatography & Related Technologies, 2003
- Antioxidant Activity of Anthocyanins and Their AglyconsJournal of Agricultural and Food Chemistry, 2003
- Induction of Apoptosis in Cancer Cells by Bilberry (Vaccinium myrtillus) and the AnthocyaninsJournal of Agricultural and Food Chemistry, 2002
- Antimutagenicity of Deacylated Anthocyanins in Purple-fleshed SweetpotatoBioscience, Biotechnology, and Biochemistry, 2001
- Suppression of Tumor Cell Growth by Anthocyanins In VitroCancer Investigation, 1995
- Use of HPLC separation/photodiode array detection for characterization of anthocyaninsJournal of Agricultural and Food Chemistry, 1990
- Food colorants: AnthocyaninsCritical Reviews in Food Science and Nutrition, 1989
- Specific colour reaction for the detection of 1,2-linked reducing disaccharides on paper and thin-layer chromatogramsJournal of Chromatography A, 1978