Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability
Open Access
- 17 December 2007
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 179 (6), 1179-1192
- https://doi.org/10.1083/jcb.200705145
Abstract
Tethering factors mediate initial interaction of transport vesicles with target membranes. Soluble N-ethylmaleimide–sensitive fusion protein attachment protein receptors (SNAREs) enable consequent docking and membrane fusion. We demonstrate that the vesicle tether conserved oligomeric Golgi (COG) complex colocalizes and coimmunoprecipitates with intra-Golgi SNARE molecules. In yeast cells, the COG complex preferentially interacts with the SNARE complexes containing yeast Golgi target (t)-SNARE Sed5p. In mammalian cells, hCog4p and hCog6p interact with Syntaxin5a, the mammalian homologue of Sed5p. Moreover, fluorescence resonance energy transfer reveals an in vivo interaction between Syntaxin5a and the COG complex. Knockdown of the mammalian COG complex decreases Golgi SNARE mobility, produces an accumulation of free Syntaxin5, and decreases the steady-state levels of the intra-Golgi SNARE complex. Finally, overexpression of the hCog4p N-terminal Syntaxin5a-binding domain destabilizes intra-Golgi SNARE complexes, disrupting the Golgi. These data suggest that the COG complex orchestrates vesicular trafficking similarly in yeast and mammalian cells by binding to the t-SNARE Syntaxin5a/Sed5p and enhancing the stability of intra-Golgi SNARE complexes.Keywords
This publication has 57 references indexed in Scilit:
- Rab6 Regulates Both ZW10/RINT-1– and Conserved Oligomeric Golgi Complex-dependent Golgi Trafficking and HomeostasisMolecular Biology of the Cell, 2007
- IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complexExperimental Cell Research, 2006
- On and Off Membrane Dynamics of the Endoplasmic Reticulum–Golgi Tethering Factor p115 In VivoMolecular Biology of the Cell, 2006
- Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7pThe EMBO Journal, 2006
- Cog1p Plays a Central Role in the Organization of the Yeast Conserved Oligomeric Golgi ComplexJournal of Biological Chemistry, 2005
- Cisternal Rab Proteins Regulate Golgi Apparatus Redistribution in Response to Hypotonic StressMolecular Biology of the Cell, 2005
- The COG and COPI Complexes Interact to Control the Abundance of GEARs, a Subset of Golgi Integral Membrane ProteinsMolecular Biology of the Cell, 2004
- Evidence for Overlapping and Distinct Functions in Protein Transport of Coat Protein Sec24p Family MembersJournal of Biological Chemistry, 2000
- Protein–Protein Interactions of the Yeast Golgi t-SNARE Sed5 Protein Distinct from Its Neural Plasma Membrane Cognate Syntaxin 1Biochemical and Biophysical Research Communications, 1998
- The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12pThe Journal of cell biology, 1997