Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine
- 2 March 2006
- journal article
- research article
- Published by Wiley in International Endodontic Journal
- Vol. 39 (5), 401-407
- https://doi.org/10.1111/j.1365-2591.2006.01094.x
Abstract
To evaluate the effect of citric acid, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminetetraacetic acid plus Cetavlon (EDTAC) solutions on the microhardness of human root canal dentine.Sixteen maxillary human canines were sectioned transversely at the cemento-enamel junction and the crowns were discarded. Subsequently, each root was embedded in an epoxy resin cylinder and their middle third sectioned horizontally into 4 mm thick slices. The samples were randomly divided into three groups according to the chelating agent employed, as follows (n = 6): group 1: EDTA 17%, group 2: EDTAC 17% and group 3: citric acid 10%. Dentine microhardness was then measured with a load of 50 g for 15 s. At the beginning of the experiment, reference microhardness values were obtained for samples without any etching (t = 0 min). The same samples were then exposed to 50 microL of the chelator solution for 1, 3 and 5 min. The Student's t-test (P < 0.05) was used to compare results for different times for each chelator and different chelators for each time.Microhardness decreased with increasing time of application of chelating solutions. There were no significant (P > 0.05) differences between initial microhardness for the three groups as well as after 1 min of application of the substances. After 3 min, EDTA produced a significantly greater reduction in microhardness. However, there was no significant difference between EDTA and EDTAC after 5 min. Citric acid caused significantly less reduction in microhardness.Overall, citric acid was least effective in reducing dentine hardness whilst EDTA had the strongest effect.Keywords
This publication has 28 references indexed in Scilit:
- Effect of EDTA and Citric Acid Solutions on the Microhardness and the Roughness of Human Root Canal DentinJournal of Endodontics, 2005
- Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasonic agitationInternational Endodontic Journal, 2002
- Effect of Hydrogen Peroxide and Sodium Perborate on Biomechanical Properties of Human DentinJournal of Endodontics, 2002
- Time-Dependent Effects of EDTA on Dentin StructuresJournal of Endodontics, 2002
- Evaluation of the Effect of EDTAC, CDTA, and EGTA on Radicular Dentin MicrohardnessJournal of Endodontics, 2001
- The influence of age and depth of dentin on bondingDental Materials, 1994
- Demineralization and Remineralization Evaluation TechniquesJournal of Dental Research, 1992
- A scanning electron microscopic evaluation of human dentinal tubules according to age and locationJournal of Endodontics, 1984
- The effect of EDTAC and the variation of its working time analyzed with scanning electron microscopyOral Surgery, Oral Medicine, Oral Pathology, 1982
- Analysis of the effect of EDTAC on the dentinal walls of the root canalJournal of Endodontics, 1977