Light-Sheet Microscopy in Neuroscience
- 8 July 2019
- journal article
- review article
- Published by Annual Reviews in Annual Review of Neuroscience
- Vol. 42 (1), 295-313
- https://doi.org/10.1146/annurev-neuro-070918-050357
Abstract
Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera. Although early implementations of light sheet were optimized for longitudinal imaging of embryonic development in small specimens, emerging implementations are capable of capturing light-sheet images in freely moving, unconstrained specimens and even the intact in vivo mammalian brain. Meanwhile, the unique photobleaching and signal-to-noise benefits afforded by light-sheet microscopy's parallelized detection deliver the ability to perform volumetric imaging at much higher speeds than can be achieved using point scanning. This review describes the basic principles and evolution of light-sheet microscopy, followed by perspectives on emerging applications and opportunities for both imaging large, cleared, and expanded neural tissues and high-speed, functional imaging in vivo.Keywords
This publication has 86 references indexed in Scilit:
- Light-Sheet Confined Super-Resolution Using Two-Photon PhotoactivationPLOS ONE, 2013
- Two-photon excitation selective plane illumination microscopy (2PE-SPIM) of highly scattering samples: characterization and applicationOptics Express, 2013
- Optimization of a GCaMP Calcium Indicator for Neural Activity ImagingJournal of Neuroscience, 2012
- Swept Field Laser Confocal Microscopy for Enhanced Spatial and Temporal Resolution in Live-Cell ImagingMicroscopy and Microanalysis, 2012
- Optical recording of action potentials in mammalian neurons using a microbial rhodopsinNature Methods, 2011
- Inverted selective plane illumination microscopy ( i SPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegansProceedings of the National Academy of Sciences of the United States of America, 2011
- Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illuminationNature Methods, 2011
- Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejectionJournal of Biomedical Optics, 2010
- Two-photon imaging with diffractive optical elementsFrontiers in Neural Circuits, 2009
- Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet MicroscopyScience, 2008