Recognition and destruction of Bacillus Calmette-Guerin-infected human monocytes.
Open Access
- 1 June 1993
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 177 (6), 1691-1698
- https://doi.org/10.1084/jem.177.6.1691
Abstract
We have established a long-term culture system to study macrophages chronically infected with mycobacteria. Monocytes are infected with Bacillus Calmette-Guerin (BCG) and support exponential intracellular replication without profound perturbation of normal host cell function. We have used this system to investigate lymphokine-activated killer (LAK)-mediated cytolysis. We have found that interleukin 2 stimulation of peripheral blood lymphocytes generates a cytotoxic activity against human monocytes. A CD56- subpopulation of LAK cells specifically recognizes and lyses BCG-infected cells. Lysis of the host cell has no effect on parasite viability and results in the liberation of bacteria capable of infecting more cells.Keywords
This publication has 15 references indexed in Scilit:
- CDlb restricts the response of human CD4−8−T lymphocytes to a microbial antigenNature, 1992
- Evidence for the involvement of CD56 molecules in alloantigen-specific recognition by human natural killer cells.The Journal of Experimental Medicine, 1991
- The systemic influence of recombinant interleukin 2 on the manifestations of lepromatous leprosy.The Journal of Experimental Medicine, 1991
- In vivo administration of low-dose human interleukin-2 induces lymphokine-activated killer cells for enhanced cytolysis in vitroCellular Immunology, 1991
- Stimulation of a major subset of lymphocytes expressing T cell receptor γδ by an antigen derived from mycobacterium tuberculosisCell, 1989
- The generation of antigen-specific, major histocompatibility complex-restricted cytotoxic T lymphocytes of the CD4+ phenotype. Enhancement by the cutaneous administration of interleukin 2.The Journal of Experimental Medicine, 1989
- The reconstitution of cell-mediated immunity in the cutaneous lesions of lepromatous leprosy by recombinant interleukin 2.The Journal of Experimental Medicine, 1989
- Killing of target cells by lymphocytes: a mechanistic view.Physiological Reviews, 1989
- Progress in the immunology of the mycobacterioses.1987
- Murine Kupffer cells. Mononuclear phagocytes deficient in the generation of reactive oxygen intermediates.The Journal of Experimental Medicine, 1985