Neural stem cell specific fluorescent chemical probe binding to FABP7
- 11 June 2012
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 109 (26), 10214-10217
- https://doi.org/10.1073/pnas.1200817109
Abstract
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.Keywords
This publication has 23 references indexed in Scilit:
- Can cellular models revolutionize drug discovery in Parkinson's disease?Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2009
- Stage- and area-specific control of stem cells in the developing nervous systemCurrent Opinion in Genetics & Development, 2009
- Fabp7 Maps to a Quantitative Trait Locus for a Schizophrenia EndophenotypePLoS Biology, 2007
- Differential development of neuronal physiological responsiveness in two human neural stem cell linesBMC Neuroscience, 2007
- Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior StageThe International Journal of Cell Cloning, 2007
- Stem cells for the treatment of neurological disordersNature, 2006
- The cell biology of neurogenesisNature Reviews Molecular Cell Biology, 2005
- Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem CellPLoS Biology, 2005
- Differentiation of mouse embryonic stem cells into a defined neuronal lineageNature Neuroscience, 2004
- Brain lipid-binding protein (BLBP): A novel signaling system in the developing mammalian CNSNeuron, 1994