Agrin is required for survival and function of monocytic cells
Open Access
- 7 June 2012
- journal article
- Published by American Society of Hematology in Blood
- Vol. 119 (23), 5502-5511
- https://doi.org/10.1182/blood-2011-09-382812
Abstract
Agrin, an extracellular matrix protein belonging to the heterogeneous family of heparan sulfate proteoglycans (HSPGs), is expressed by cells of the hematopoietic system but its role in leukocyte biology is not yet clear. Here we demonstrate that agrin has a crucial, nonredundant role in myeloid cell development and functions. We have identified lineage-specific alterations that affect maturation, survival and properties of agrin-deficient monocytic cells, and occur at stages later than stem cell precursors. Our data indicate that the cell-autonomous signals delivered by agrin are sensed by macrophages through the α-DC (DG) receptor and lead to the activation of signaling pathways resulting in rearrangements of the actin cytoskeleton during the phagocytic synapse formation and phosphorylation of extracellular signal-regulated kinases (Erk 1/2). Altogether, these data identify agrin as a novel player of innate immunity.This publication has 42 references indexed in Scilit:
- The critical role of agrin in the hematopoietic stem cell nicheBlood, 2011
- Activation of the innate immune receptor Dectin-1 upon formation of a ‘phagocytic synapse’Nature, 2011
- Modulation of Agrin-induced Acetylcholine Receptor Clustering by Extracellular Signal-regulated Kinases 1 and 2 in Cultured MyotubesOnline Journal of Public Health Informatics, 2010
- Pbx1 Regulates Self-Renewal of Long-Term Hematopoietic Stem Cells by Maintaining Their QuiescenceCell Stem Cell, 2008
- MuSK controls where motor axons grow and form synapsesNature Neuroscience, 2007
- Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromataJCI Insight, 2007
- Monocyte and macrophage heterogeneityNature Reviews Immunology, 2005
- Agrin Regulates Rapsyn Interaction with Surface Acetylcholine Receptors, and This Underlies Cytoskeletal Anchoring and ClusteringOnline Journal of Public Health Informatics, 2003
- Characterization of the β-Dystroglycan–Growth Factor Receptor 2 (Grb2) InteractionBiochemical and Biophysical Research Communications, 2000
- Differential Distribution of Agrin Isoforms in the Developing and Adult Avian RetinaMolecular and Cellular Neuroscience, 1997