Non-coding chloroplast DNA for plant molecular systematics at the infrageneric level
- 1 January 1994
- book chapter
- other
- Published by Springer Science and Business Media LLC
- Vol. 69, 391-403
- https://doi.org/10.1007/978-3-0348-7527-1_23
Abstract
With primers constructed against highly conserved regions of tRNA genes (trnT UGU, trnL UAA and trnF GAA) in chloroplast DNA, we have amplified two different non-coding spacers and one intron from four species within the genus Echium L. (Boraginaceae) and from two confamilial outgroups. The trnT UGU-trnL UAA intergenic spacer contains a greater number of polymorphic sites than the trnL UAA intron or the trnL UAA-trnF GAA intergenic spacer. We analyzed a total of 11 kb of sequence data from this non-coding DNA. Total nucleotide divergence between Echium species is on the order of 1% for these regions, all of which possess infrageneric length polymorphisms. The latter two regions contain indels which occur only in the 14 Macaronesian Island endemic species of Echium studied and suggest that these may form a monophyletic group.Keywords
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