CDKN2A/p16 in ependymomas
- 1 January 2001
- journal article
- Published by Springer Science and Business Media LLC in Journal of Neuro-Oncology
- Vol. 54 (1), 9-13
- https://doi.org/10.1023/a:1012537105775
Abstract
Sixteen cases of ependymoma were studied for CDKN2A/p16 inactivation by immunohistochemistry using a p16 monoclonal antibody, by homozygous deletion (HD) assay and 5′CpG promoter methylation assay (methylation-specific PCR). Three out of 16 cases were p16 immuno-negative: two corresponded to grade II ependymomas and one to grade III. The latter ependymoma, characterized by a high Ki-67/MIB-1 LI, was the only one of the whole series to show CDKN2A HD. No promoter methylation was found in the two immuno-negative cases without CDKN2A HD. Alternative mechanisms, such as point mutations or alterations in p16 post-translational regulation, may be responsible for p16 inactivation. Since in our series just one out of eight anaplastic cases showed negative immunostaining and CDKN2A HD, p16/CDKN2A inactivation may not play an important role in the malignant transformation of ependymomas. Amplification of CCND1 and CDK4, p27/Kip1 degradation and TP53 mutations were previously studied by other authors and were demonstrated not to correlate with anaplasia. Up to date, molecular genetic studies have not been useful in recognizing the anaplastic variant in ependymomas.Keywords
This publication has 23 references indexed in Scilit:
- p16INK4a and p19INK4d mRNA expression in neuroglial tumours: correlation with Ki67 proliferation indexNeuropathology and Applied Neurobiology, 1999
- CDKN2/p16 Predicts Survival in Oligodendrogliomas: Comparison with AstrocytomasJournal of Neuro-Oncology, 1999
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences of the United States of America, 1996
- Silencing of p16/CDKN2 expression in human gliomas by methylation and chromatin condensation.1996
- Homozygous deletions of the multiple tumor suppressor gene 1 in the progression of human astrocytomas.1995
- CDKN2 (p16/MTS1) gene deletion or CDK4 amplification occurs in the majority of glioblastomas.1994
- Mutations of the p53 tumor suppressor gene in neoplasms of the human nervous systemMolecular Carcinogenesis, 1993
- Ependymoma: Internal Correlations among Pathological Signs: The Anaplastic VariantNeurosurgery, 1991
- Lack of histopathological correlation of malignant ependymomas with postoperative survivalJournal of Neurosurgery, 1989
- Ependymoma.A follow-up study of 101 casesCancer, 1977