Cholesterol biosensor based on rf sputtered zinc oxide nanoporous thin film
- 6 August 2007
- journal article
- research article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 91 (6), 063901
- https://doi.org/10.1063/1.2768302
Abstract
Cholesterol oxidase (ChOx) has been immobilized onto zinc oxide (ZnO) nanoporous thin films grown on gold surface. A preferred -axis oriented ZnO thin film with porous surface morphology has been fabricated by rf sputtering under high pressure. Optical studies and cyclic voltammetric measurements show that the bioelectrode is sensitive to the detection of cholesterol in range. A relatively low value of enzyme’s kinetic parameter (Michaelis-Menten constant) indicates enhanced enzyme affinity of ChOx to cholesterol. The observed results show promising application of nanoporous ZnO thin film for biosensing application without any functionalization.
Keywords
This publication has 22 references indexed in Scilit:
- Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase ActivityLangmuir, 2007
- Development of a screen-printed cholesterol biosensor: Comparing the performance of gold and platinum as the working electrode material and fabrication using a self-assembly approachSensors and Actuators B: Chemical, 2007
- Low Frequency Raman Scattering from Acoustic Phonons Confined in ZnO NanoparticlesPhysical Review Letters, 2006
- Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance techniqueTalanta, 2006
- Examination of Cholesterol oxidase attachment to magnetic nanoparticlesJournal of Nanobiotechnology, 2005
- Nanogold-plasmon-resonance-based glucose sensingAnalytical Biochemistry, 2004
- Amperometric cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on conducting polypyrrole filmsAnalytica Chimica Acta, 2004
- An electrochemical multienzymatic biosensor for determination of cholesterolBioelectrochemistry, 2001
- Co-immobilization of cholesterol oxidase and horseradish peroxidase in a sol–gel filmAnalytica Chimica Acta, 2000
- Bioelectrochemical characteristics of cholesterol oxidase immobilized in a polyaniline filmSensors and Actuators B: Chemical, 1999