Slit and glypican‐1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain
- 6 March 2003
- Vol. 42 (2), 130-138
- https://doi.org/10.1002/glia.10207
Abstract
The slit family serves as a repellent for growing axons toward correct targets during neural development. A recent report describes slit mRNAs expressed in various brain regions in adult rats. However, their functions in the adult nervous system remain unknown. In the present study, we investigated whether slit mRNAs were expressed in the cryo‐injured brain, using in situ hybridization. All slit family members were expressed at the lesion. Slit2 mRNA was the most intensely expressed in the cells surrounding the necrotic tissue. A double‐labeling study showed that slit2 mRNA was expressed in the glial fibrillary acidic protein (GFAP)‐positive reactive astrocytes. In addition, glypican‐1, a heparan sulfate proteoglycan that serves as a high‐affinity receptor for Slit protein, was coexpressed with slit2 mRNA in the reactive astrocytes. These findings suggested that slit2 might prevent regenerating axons from entering into the lesion in concert with glypican‐1. GLIA 42:130–138, 2003.Keywords
This publication has 41 references indexed in Scilit:
- Increased syndecan expression by pleiotrophin and FGF receptor‐expressing astrocytes in injured brain tissueGlia, 2002
- Spatiotemporal expression patterns of slit and robo genes in the rat brainJournal of Comparative Neurology, 2001
- Characterization of Slit Protein Interactions with Glypican-1Published by Elsevier BV ,2001
- The Expression of TAPA (CD81) Correlates with the Reactive Response of Astrocytes in the Developing Rat CNSExperimental Neurology, 1999
- Intracranial Injury Acutely Induces the Expression of the Secreted Isoform of the CNS-Specific Hyaluronan-Binding Protein BEHAB/BrevicanExperimental Neurology, 1999
- Mammalian Homologues of the Drosophila Slit Protein Are Ligands of the Heparan Sulfate Proteoglycan Glypican-1 in BrainPublished by Elsevier BV ,1999
- A Novel Geneiba1in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic LineageBiochemical and Biophysical Research Communications, 1996
- Recovery from spinal cord injury mediated by antibodies to neurite growth inhibitorsNature, 1995
- Sprouting and Regeneration of Lesioned Corticospinal Tract Fibres in the Adult Rat Spinal CordEuropean Journal of Neuroscience, 1993
- Axonal regeneration in the rat spinal cord produced by an antibody against myelin-associated neurite growth inhibitorsNature, 1990