Role of Local Immunoglobulin E Specific for Alternaria alternata in the Pathogenesis of Nasal Polyposis
- 2 January 2008
- journal article
- research article
- Published by Wiley in The Laryngoscope
- Vol. 118 (1), 4-9
- https://doi.org/10.1097/mlg.0b013e3181567a7a
Abstract
The role of fungal pathogens in the etiology of nasal polyposis remains unclear. The aim of this study was to determine whether there was a correlation between the presence of Alternaria-specific immunoglobulin (Ig)E antibodies, eosinophilic inflammation, and the development of nasal polyps. Prospective study. Serum and nasal tissue homogenates from 21 patients with manifestations of chronic sinusitis with nasal polyps were compared with specimens from 13 chronic sinusitis patients without polyps and 8 healthy controls. The Phadia ImmunoCAP and enzyme-linked immunosorbent assay were used to quantify levels of total IgE and Alternaria-specific (IgE, IgG, and IgA) antibodies. Eosinophil cationic protein (ECP) and tryptase levels were measured in tissue homogenates, whereas the inflammatory response was evaluated using tissue eosinophil counts in tissue samples. Serum analysis revealed no difference in the levels of total IgE and Alternaria-specific IgE, IgG, and IgA antibodies between the study groups. In contrast, the levels of Alternaria-specific IgE in tissue with polyps were significantly higher than in nonpolyp tissue. Increases in total tissue IgE paralleled increased levels of Alternaria-specific IgG and IgA antibodies in chronic sinusitis with nasal polyps as compared with control groups. A positive correlation was found between Alternaria-specific IgE and ECP in tissue. Increased mean levels of ECP corresponded to increased eosinophil counts in the group of patients with polyps. Alternaria-specific IgE and eosinophilic inflammation in nasal tissue correlates with the incidence of nasal polyps irrespective of specific IgE antibodies in serum. Together, the correlation between the local immune responses and the eosinophilic inflammation in nasal polyps suggests a possible role of Alternaria in the pathogenesis of nasal polyposis.Keywords
This publication has 17 references indexed in Scilit:
- Rhinosinusitis: Developing guidance for clinical trialsJournal of Allergy and Clinical Immunology, 2006
- Fungal-Specific Humoral Response in Eosinophilic Mucus Chronic RhinosinusitisThe Laryngoscope, 2005
- Rhinosinusitis: Establishing definitions for clinical research and patient careJournal of Allergy and Clinical Immunology, 2004
- Chronic rhinosinusitis: An enhanced immune response to ubiquitous airborne fungiJournal of Allergy and Clinical Immunology, 2004
- Pathogenesis of chronic rhinosinusitis: role of airborne fungi and bacteriaImmunology and Allergy Clinics of North America, 2004
- Fungal DNA is Present in Tissue Specimens of Patients with Chronic RhinosinusitisAmerican Journal of Rhinology, 2004
- Editors and Reviewers: Supporting Peer-Review PublishingOtolaryngology -- Head and Neck Surgery, 2003
- Measurement of Inflammatory Mediators of Mast Cells and Eosinophils in Native Nasal Lavage Fluid in Nasal PolyposisInternational Archives of Allergy and Immunology, 2001
- Total and specific IgE in nasal polyps is related to local eosinophilic inflammationJournal of Allergy and Clinical Immunology, 2001
- The Diagnosis and Incidence of Allergic Fungal SinusitisMayo Clinic Proceedings, 1999