Powering through ribosome assembly
- 22 October 2009
- journal article
- review article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 15 (12), 2083-2104
- https://doi.org/10.1261/rna.1792109
Abstract
Ribosome assembly is required for cell growth in all organisms. Classic in vitro work in bacteria has led to a detailed understanding of the biophysical, thermodynamic, and structural basis for the ordered and correct assembly of ribosomal proteins on ribosomal RNA. Furthermore, it has enabled reconstitution of active subunits from ribosomal RNA and proteins in vitro. Nevertheless, recent work has shown that eukaryotic ribosome assembly requires a large macromolecular machinery in vivo. Many of these assembly factors such as ATPases, GTPases, and kinases hydrolyze nucleotide triphosphates. Because these enzymes are likely regulatory proteins, much work to date has focused on understanding their role in the assembly process. Here, we review these factors, as well as other sources of energy, and their roles in the ribosome assembly process. In addition, we propose roles of energy-releasing enzymes in the assembly process, to explain why energy is used for a process that occurs largely spontaneously in bacteria. Finally, we use literature data to suggest testable models for how these enzymes could be used as targets for regulation of ribosome assembly.Keywords
This publication has 150 references indexed in Scilit:
- Mutation of a Gene Essential for Ribosome Biogenesis, EMG1, Causes Bowen-Conradi SyndromeAmerican Journal of Human Genetics, 2009
- 18S rRNA processing requires base pairings of snR30 H/ACA snoRNA to eukaryote-specific 18S sequencesThe EMBO Journal, 2009
- A Genetic Interaction Map of RNA-Processing Factors Reveals Links between Sem1/Dss1-Containing Complexes and mRNA Export and SplicingMolecular Cell, 2008
- Differential RNA-Dependent ATPase Activities of Four rRNA Processing Yeast DEAD-Box ProteinsBiochemistry, 2008
- Structure of the Mammalian 80S Ribosome at 8.7 Å ResolutionStructure, 2008
- Rapid, Transcript-Specific Changes in Splicing in Response to Environmental StressMolecular Cell, 2007
- Monopolar Attachment of Sister Kinetochores at Meiosis I Requires Casein Kinase 1Cell, 2006
- Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometryNature, 2002
- Functional organization of the yeast proteome by systematic analysis of protein complexesNature, 2002
- The GTPase superfamily: conserved structure and molecular mechanismNature, 1991