Enhancement of Umbilical Cord Blood Cell Hematopoiesis by Maitake Beta-Glucan Is Mediated by Granulocyte Colony-Stimulating Factor Production
Open Access
- 1 January 2007
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Vaccine Immunology
- Vol. 14 (1), 21-27
- https://doi.org/10.1128/cvi.00284-06
Abstract
Maitake beta-glucan (MBG) is an extract from the fruit body of theGrifola frondosamushroom that is being widely used to treat cancer in Asia. We have previously reported that MBG enhances mouse bone marrow cell (BMC) hematopoiesis in vitro and protects BMC from doxorubicin (DOX) toxicity. In the current study, we investigated the ability of MBG to enhance hematopoiesis and to reduce the toxic effects of DOX on fresh human umbilical cord blood (CB) cells. MBG treatment significantly enhanced the colony formation unit (CFU) response of granulocytes-macrophages (CFU-GM response) over the whole dose range of 12.5 to 100 μg/ml (P< 0.05). The addition of MBG to DOX-treated CB cells significantly protected granulocyte-macrophage colony formation from the toxicity of DOX, which otherwise produced strong hematopoietic repression. MBG also partially replaced recombinant human granulocyte colony-stimulating factor (rhG-CSF), as shown by a significant augmentation of the CFU-GM response in the absence of rhG-CSF. We found that MBG induces granulocyte colony-stimulating factor (G-CSF) production in CB CD33+monocytes, as detected by intracellular cytokine flow cytometric assessment. In contrast, we found that adult peripheral blood monocytes did not produce a significant G-CSF response to MBG, whereas both adult and CB monocytes produced G-CSF in response to lipopolysaccharide. These studies provide the first evidence that MBG induces hematopoietic stem cell proliferation and differentiation of CFU-GM in umbilical CB cells and acts directly to induce G-CSF.Keywords
This publication has 45 references indexed in Scilit:
- β-Glucan enhances complement-mediated hematopoietic recovery after bone marrow injuryBlood, 2006
- Relationship between dendritic cells and the D-fraction-induced Th-1 dominant response in BALB/c tumor-bearing miceCancer Letters, 2003
- Exposure to Particulate 1→3-β-Glucans Induces Greater Pulmonary Toxicity Than Soluble 1→3-β-Glucans in RatsJournal of Toxicology and Environmental Health, Part A, 2003
- Dectin-1 Is A Major β-Glucan Receptor On MacrophagesThe Journal of Experimental Medicine, 2002
- Mobilization of Peripheral Blood Progenitor Cells by Betafectin® PGG‐Glucan Alone and in Combination with Granulocyte Colony‐Stimulating FactorThe International Journal of Cell Cloning, 1998
- Enhancement of hematopoietic response of mice by intraperitoneal administration of a .BETA.-glucan, SSG, obtained from Sclerotinia sclerotiorum.Journal of Pharmacobio-Dynamics, 1990
- Antitumor activity exhibited by orally administered extract from fruit body of Grifola frondosa (Maitake).CHEMICAL & PHARMACEUTICAL BULLETIN, 1988
- The Chemical Structure of an Antitumor Polysaccharide in Fruit Bodies of Grifola frondosa (Maitake)CHEMICAL & PHARMACEUTICAL BULLETIN, 1987
- Potentiation of host-mediated antitumor activity in mice by .BETA.-glucan obtained from Grifola frondosa (maitake)CHEMICAL & PHARMACEUTICAL BULLETIN, 1987
- Stimulated hemopoiesis and enhanced survival following glucan treatment in sublethally and lethally irradiated miceInternational Journal of Immunopharmacology, 1985