Role of Absolute Humidity in the Inactivation of Influenza Viruses on Stainless Steel Surfaces at Elevated Temperatures
- 15 June 2010
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 76 (12), 3943-3947
- https://doi.org/10.1128/aem.02674-09
Abstract
Influenza virus has been found to persist in the environment for hours to days, allowing for secondary transmission of influenza via inanimate objects known as fomites. We evaluated the efficacy of heat and moisture for the decontamination of surfaces for the purpose of preventing of the spread of influenza. Aqueous suspensions of influenza A virus were deposited onto stainless steel coupons, allowed to dry under ambient conditions, and exposed to temperatures of 55°C, 60°C, or 65°C and relative humidity (RH) of 25%, 50%, or 75% for up to 1 h. Quantitative virus assays were performed on the solution used to wash the viruses from these coupons, and results were compared with the solution used to wash coupons treated similarly but left under ambient conditions. Inactivation of influenza virus on surfaces increased with increasing temperature, RH, and exposure time. Reductions of greater than 5 logs of influenza virus on surfaces were achieved at temperatures of 60 and 65°C, exposure times of 30 and 60 min, and RH of 50 and 75%. Our data also suggest that absolute humidity is a better predictor of surface inactivation than RH and allows the prediction of survival using two parameters rather than three. Modest amounts of heat and adequate moisture can provide effective disinfection of surfaces while not harming surfaces, electrical systems, or mechanical components, leaving no harmful residues behind after treatment and requiring a relatively short amount of time.Keywords
This publication has 27 references indexed in Scilit:
- The effect of environmental parameters on the survival of airborne infectious agentsJournal of The Royal Society Interface, 2009
- Emergence of a Novel Swine-Origin Influenza A (H1N1) Virus in HumansNew England Journal of Medicine, 2009
- Absolute humidity modulates influenza survival, transmission, and seasonalityProceedings of the National Academy of Sciences of the United States of America, 2009
- Inactivation of influenza A viruses in the environment and modes of transmission: A critical reviewJournal of Infection, 2008
- High Temperature (30°C) Blocks Aerosol but Not Contact Transmission of Influenza VirusJournal of Virology, 2008
- Influenza Virus Transmission Is Dependent on Relative Humidity and TemperaturePLoS Pathogens, 2007
- Inactivation of Influenza A Virus on Copper versus Stainless Steel SurfacesApplied and Environmental Microbiology, 2007
- Significance of Fomites in the Spread of Respiratory and Enteric Viral DiseaseApplied and Environmental Microbiology, 2007
- How Contagious Are Common Respiratory Tract Infections?New England Journal of Medicine, 2003
- Survival of airborne influenza virus: Effects of propagating host, relative humidity, and composition of spray fluidsArchiv für die gesamte Virusforschung, 1976