High-performance liquid chromatographic analysis of ginsenosides inPanax ginseng extracts using glass-ODS column
- 1 December 1987
- journal article
- research article
- Published by Springer Science and Business Media LLC in Chromatographia
- Vol. 24 (1), 517-519
- https://doi.org/10.1007/bf02688537
Abstract
Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 µg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.Keywords
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