BMP signals and the transcriptional repressor BLIMP1 during germline segregation in the mammalian embryo
Open Access
- 1 September 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 221 (4), 209-223
- https://doi.org/10.1007/s00427-011-0373-5
Abstract
Molecular factors and tissue compartments involved in the foundation of the mammalian germline have been mainly described in the mouse so far. To find mechanisms applicable to mammals in general, we analyzed temporal and spatial expression patterns of the transcriptional repressor BLIMP1 (also known as PRDM1) and the signaling molecules BMP2 and BMP4 in perigastrulation and early neurulation embryos of the rabbit using whole-mount in situ hybridization and high-resolution light microscopy. Both BMP2 and BMP4 are expressed in annular domains at the boundary of the embryonic disc, which—in contrast to the situation in the mouse—partly belong to intraembryonic tissues. While BMP2 expression begins at (pregastrulation) stage 1 in the hypoblast, BMP4 expression commences—distinctly delayed compared to the mouse—diffusely at (pregastrulation) stage 2; from stage 3 onwards, BMP4 is expressed peripherally in hypoblast and epiblast and in the mesoderm at the posterior pole of the embryonic disc. BLIMP1 expression begins throughout the hypoblast at stage 1 and emerges in single primordial germ cell (PGC) precursors in the posterior epiblast at stage 2 and then in single mesoderm cells at positions identical to those identified by PGC-specific antibodies. These expression patterns suggest that function and chronology of factors involved in germline segregation are similar in mouse and rabbit, but higher temporal and spatial resolution offered by the rabbit demonstrates a variable role of bone morphogenetic proteins and makes “blimping” a candidate case for lateral inhibition without the need for an allantoic germ cell niche.Keywords
This publication has 52 references indexed in Scilit:
- BMP signaling controls formation of a primordial germ cell niche within the early genital ridgesDevelopmental Biology, 2010
- Developmental Stages in Human Embryos: Revised and New MeasurementsCells Tissues Organs, 2010
- Whole-Mount In Situ Immunofluorescence of Early Rabbit EmbryosCold Spring Harbor Protocols, 2010
- Mouse TEX14 Is Required for Embryonic Germ Cell Intercellular Bridges but Not Female Fertility1Biology of Reproduction, 2009
- Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in miceGenes & Development, 2008
- Subcellular Redistribution of the Mitochondrial PG2 Epitope during Development from Cleavage to Primordial Germ Cell Formation in the Rabbit EmbryoSexual Development, 2008
- Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursorsBMC Developmental Biology, 2007
- Gene Expression Dynamics During Germline Specification in Mice Identified by Quantitative Single-Cell Gene Expression Profiling1Biology of Reproduction, 2006
- Requirement of Bmp8b for the Generation of Primordial Germ Cells in the MouseMolecular Endocrinology, 2000
- Colocalization of BMP 7 and BMP 2 RNAs suggests that these factors cooperatively mediate tissue interactions during murine developmentMechanisms of Development, 1995