Adaptation of an in‐house PCR for the detection of Helicobacter pylori and the mutations associated with macrolide resistance into ready‐to‐use PCR microwell strips
- 10 October 2021
- journal article
- research article
- Published by Wiley in Helicobacter
- Vol. 26 (6), e12855
- https://doi.org/10.1111/hel.12855
Abstract
Background and Objectives The present study describes the successful adaptation of an in-house Polymerase Chain Reaction (PCR) for Helicobacter pylori detection coupled with the main mutations associated with resistance to clarithromycin in ready-to-use PCR microwell strips. Materials and Methods These microwell strips can be used on LightCycler® 480, and are delivered with nine microliters of the reaction mixture dispensed into 8-well microwell strips. An extraction control PCR targeting the β-globin household gene is amplified in the same run as H pylori detection. Results and Conclusion These microwell strips can be stored at −20°C for 1 year and left at room temperature and in the light for up to 4 h with no impact on the PCR results. Microwell strips can also undergo a thaw and refreeze cycle without impacting the PCR results. These PCR microwell strips are available for purchase from Eurogentec.Keywords
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