Broadening of DNA replication origin usage during metazoan cell differentiation
- 16 June 2006
- journal article
- Published by EMBO in EMBO Reports
- Vol. 7 (8), 806-811
- https://doi.org/10.1038/sj.embor.7400736
Abstract
We have examined whether replication of the chicken β‐globin locus changes during differentiation of primary erythroid progenitors into erythrocytes. In undifferentiated progenitors, four principal initiation sites and a replication fork pausing region (RFP) were observed. Forty‐eight hours after induction of differentiation, the principal sites were maintained, even in the activated βA‐globin gene, some minor sites were enhanced, three new sites appeared and the RFP disappeared. One of the activated origins showed increased histone H3 K9K14 diacetylation, but the others did not. These results demonstrate a broadening of DNA replication origin usage during differentiation of untransformed metazoan cells and indicate that histone H3 diacetylation, other histone modifications so far reported and transcription are not crucial determinants of origin selection in this system.Keywords
This publication has 22 references indexed in Scilit:
- Origins Go PlasticMolecular Cell, 2005
- Progressive Activation of DNA Replication Initiation in Large Domains of the Immunoglobulin Heavy Chain Locus during B Cell DevelopmentMolecular Cell, 2005
- The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chickenNucleic Acids Research, 2005
- Methylation of histone H4 by arginine methyltransferase PRMT1 is essential in vivo for many subsequent histone modificationsGenes & Development, 2005
- Specific signals at the 3′ end of the DHFR gene define one boundary of the downstream origin of replicationGenes & Development, 2005
- Coordination of replication and transcription along a Drosophila chromosomeGenes & Development, 2004
- Replication Initiation Patterns in the β-Globin Loci of Totipotent and Differentiated Murine Cells: Evidence for Multiple Initiation RegionsMolecular and Cellular Biology, 2002
- Correlation Between Histone Lysine Methylation and Developmental Changes at the Chicken β-Globin LocusScience, 2001
- Transitions in histone acetylation reveal boundaries of three separately regulated neighboring lociThe EMBO Journal, 2001
- Developmental regulation of replication fork pausing in Xenopus laevis ribosomal RNA genesJournal of Molecular Biology, 1999