Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model
Open Access
- 1 August 2004
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (8), 4911-4917
- https://doi.org/10.1128/iai.72.8.4911-4917.2004
Abstract
Brucella abortus 2308 derivatives with mini-Tn 5 insertions in purE , purL , and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn 5 insertions in pheA , trpB , and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment.Keywords
This publication has 21 references indexed in Scilit:
- The analysis of the intramacrophagic virulome ofBrucella suisdeciphers the environment encountered by the pathogen inside the macrophage host cellProceedings of the National Academy of Sciences of the United States of America, 2002
- Brucella Infections and ImmunityPublished by Springer Science and Business Media LLC ,2002
- The genome sequence of the facultative intracellular pathogen Brucella melitensisProceedings of the National Academy of Sciences of the United States of America, 2001
- Aromatic Compound-Dependent Brucella suis Is Attenuated in Both Cultured Cells and Mouse ModelsInfection and Immunity, 2001
- Similar Requirements of a Plant Symbiont and a Mammalian Pathogen for Prolonged Intracellular SurvivalScience, 2000
- A two‐component regulatory system playing a critical role in plant pathogens and endosymbionts is present in Brucella abortus and controls cell invasion and virulenceMolecular Microbiology, 1998
- Mutagenesis of Brucella abortus: comparative efficiency of three transposon delivery systemsMicrobial Pathogenesis, 1991
- Intracellular replication is essential for the virulence of Salmonella typhimurium.Proceedings of the National Academy of Sciences of the United States of America, 1991
- Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent.Proceedings of the National Academy of Sciences of the United States of America, 1986
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences of the United States of America, 1977