Identification of a Transcriptional Enhancer Element Upstream from the Proto-Oncogene fos
- 6 December 1985
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 230 (4730), 1174-1177
- https://doi.org/10.1126/science.3865371
Abstract
Sequences upstream from the proto-oncogene fos were shown to be essential for its transcription. Transient expression of the chloramphenicol acetyl-transferase (CAT) gene linked to upstream sequences of the fos gene including its promoter reveals that sequences located 64 to 404 base pairs 5' to the fos cap site contain a typical transcriptional enhancer. Moreover, these enhancer sequences, which are strikingly conserved between mouse and human fos genes, coincide with a deoxyribonuclease I-hypersensitive site in the chromatin. The expression of the fos-CAT fusion genes was stimulated only two to three times by the fos inducer 12-0-tetradecanoyl phorbol-13-acetate. The fos enhancer does not appear to be tissue-specific.Keywords
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