Pineal‐gonadal relationship in the teleost Channa punctatus (Bloch): Evidence for possible involvement of hypothalamic serotonergic system

Abstract

In Channa punctatus, pinealectomy results in an accelerated growth (stimulatory effect) of the ovary in preparatory phase (March), but had no significant effect in prespawning (May-June) or postspawning (September) phases. Administration of melatonin (25 micrograms/fish or 0.4 mg/kg BW at 2-day intervals for 30 days) inhibits the stimulatory effect of a long photoperiod and high temperature (16L:8D; 22 +/- 1 degree C) on the ovary in the early preparatory phase (February). In the late preparatory phase (April), the administration of both melatonin (0.5 mg/kg BW at 2-day intervals) and parachlorophenylalanine (pCPA, a serotonin synthesis blocker; 100 mg/kg BW at 3-day intervals) for 30 days inhibits ovarian activity in comparison to that of the saline-treated control group. In the dose-response study, greater than or equal to 0.5 mg/kg BW of melatonin induced a significant increase in hypothalamic 5-HT content and greater than or equal to 10 mg/kg BW of pCPA decreased it significantly. In the time-course study, melatonin (2.0 mg/kg BW) elevates the 5-HT content significantly after an hr of the injection and maintained it up to 48 hrs. The administration of pCPA (200 mg/kg BW) had significantly inhibited the 5-HT content which was sustained for 72 hr. In another study, a single injection of melatonin (0.5 mg/kg BW) increased the 5-HT content significantly. A single injection of pCPA (100 mg/kg BW) decreased significantly both the content and activity of 5-HT. It is inferred that hypothalamic 5-HT may play a central role in photosexual mechanisms and mediate long photoperiodic effects on neuroendocrine-reproductive axis.