Evaluation of a 3% surf solution (surf field mastitis test) for the diagnosis of subclinical bovine and bubaline mastitis
- 3 September 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Tropical Animal Health and Production
- Vol. 42 (3), 457-464
- https://doi.org/10.1007/s11250-009-9443-3
Abstract
To evaluate a 3% solution of household detergent viz., Surf Excel (Surf field mastitis test, SFMT) vis-à-vis California mastitis test (CMT), Whiteside test (WST), somatic cell counts (SCC; cut off limit = 5 × 105 cells per millilitre) and bacteriological cultures for the detection of subclinical mastitis in quarter foremilk samples (n = 800) of dairy cows and buffaloes. Culture and SCC were used as gold standards. All tests were evaluated parallel and serial patterns. The sensitivities of SFMT, SCC, culture, CMT and WST in parallel testing were 72.82, 81.55, 87.38, 75.73 and 54.37%, respectively in cows, while 66.22, 79.73, 82.43, 70.27 and 50.00, respectively in buffaloes. SFMT was significantly (p < 0.05) more sensitive than WST and comparable to CMT in both species. In serial testing, percent specificity of SFMT (87.12 in cow; 85.16 in buffaloes) was significantly (P < 0.05) higher than that of CMT (83.33 in cow; 80.64 in buffaloes). The negative predictive values of SFMT (93.50 in cow; 96.35 in buffaloes) differed non-significantly from that of CMT (94.02 in cow; 96.15 in buffaloes). The kappa index between the tests was moderate to perfect both in parallel (0.54 to >0.80) and serial (0.58 to >0.8) testing. On the basis of closely similar diagnostic efficiency of SFMT to CMT in terms of sensitivity, specificity, predictive values and kappa index together with inexpensive and ready availability of SFMT reagent, it tempting to suggest that SFMT can be use as a cheaper, user-friendly alternative animal-side subclinical mastitis diagnostic test in poor countries.Keywords
This publication has 14 references indexed in Scilit:
- Bovine mastitis and its association with selected risk factors in smallholder dairy farms in and around Bahir Dar, EthiopiaTropical Animal Health and Production, 2007
- Bovine mastitis and antibiotic resistance patterns in Selalle smallholder dairy farms, central EthiopiaTropical Animal Health and Production, 2007
- Comparative Evaluation of Rose Bengal Plate Agglutination Test, Mallein Test, and Some Conventional Serological Tests for Diagnosis of Equine GlandersJournal of Veterinary Diagnostic Investigation, 2007
- Effect of mastitis on raw milk compositional qualityJournal of Veterinary Science, 2007
- Understanding interobserver agreement: the kappa statistic.2005
- Assessment of the California mastitis test usage in smallholder dairy herds and risk of violative antimicrobial residuesJournal of Veterinary Science, 2004
- Indicators of inflammation in the diagnosis of mastitisVeterinary Research, 2003
- Mastitis therapy and pharmacologyVeterinary Clinics of North America: Food Animal Practice, 2003
- Comparison of Some Screening Tests for Detecting MastitisJournal of Dairy Science, 1987
- EVALUATION OF TESTS1977