Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice
Open Access
- 22 December 2016
- journal article
- Published by Hindawi Limited in Neural Plasticity
- Vol. 2016, 1-16
- https://doi.org/10.1155/2016/6720420
Abstract
MYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-induced hearing loss (NIHL) susceptible gene. However, the specific roles of MYH14 in auditory function and NIHL are not fully understood. In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14−/−mice) and clarify the role of MYH14 in the cochlea and NIHL. We found that Myh14−/−mice did not exhibit significant hearing loss until five months of age. In addition, Myh14−/−mice were more vulnerable to high intensity noise compared to control mice. More significant outer hair cell loss was observed in Myh14−/−mice than in wild type controls after acoustic trauma. Our findings suggest that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice.Keywords
Funding Information
- National Natural Science Foundation of China (31171194, 2014CB541703, 2009GG10002039)
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