A LuxS-Dependent Cell-to-Cell Language Regulates Social Behavior and Development inBacillus subtilis
- 15 June 2006
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 188 (12), 4442-4452
- https://doi.org/10.1128/jb.00165-06
Abstract
Cell-to-cell communication in bacteria is mediated by quorum-sensing systems (QSS) that produce chemical signal molecules called autoinducers (AI). In particular, LuxS/AI-2-dependent QSS has been proposed to act as a universal lexicon that mediates intra- and interspecific bacterial behavior. Here we report that the model organism Bacillus subtilis operates a luxS-dependent QSS that regulates its morphogenesis and social behavior. We demonstrated that B. subtilis luxS is a growth-phase-regulated gene that produces active AI-2 able to mediate the interspecific activation of light production in Vibrio harveyi. We demonstrated that in B. subtilis, luxS expression was under the control of a novel AI-2-dependent negative regulatory feedback loop that indicated an important role for AI-2 as a signaling molecule. Even though luxS did not affect spore development, AI-2 production was negatively regulated by the master regulatory proteins of pluricellular behavior, SinR and Spo0A. Interestingly, wild B. subtilis cells, from the undomesticated and probiotic B. subtilis natto strain, required the LuxS-dependent QSS to form robust and differentiated biofilms and also to swarm on solid surfaces. Furthermore, LuxS activity was required for the formation of sophisticated aerial colonies that behaved as giant fruiting bodies where AI-2 production and spore morphogenesis were spatially regulated at different sites of the developing colony. We proposed that LuxS/AI-2 constitutes a novel form of quorum-sensing regulation where AI-2 behaves as a morphogen-like molecule that coordinates the social and pluricellular behavior of B. subtilis.Keywords
This publication has 64 references indexed in Scilit:
- LuxS controls bacteriocin production in Streptococcus mutans through a novel regulatory componentMolecular Microbiology, 2005
- TheBacillus subtilisSinR and RapA Developmental Regulators Are Responsible for Inhibition of Spore Development by AlcoholJournal of Bacteriology, 2005
- Sociomicrobiology: the connections between quorum sensing and biofilmsTrends in Microbiology, 2005
- Chemical communication in proteobacteria: biochemical and structural studies of signal synthases and receptors required for intercellular signallingMolecular Microbiology, 2004
- Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motilityMolecular Microbiology, 2004
- Novel Roles of the Master Transcription Factors Spo0A and σBfor Survival and Sporulation ofBacillus subtilisat Low Growth TemperatureJournal of Bacteriology, 2004
- Mutation ofluxSAffects Biofilm Formation inStreptococcus mutansInfection and Immunity, 2003
- Listening in on bacteria: acyl-homoserine lactone signallingNature Reviews Molecular Cell Biology, 2002
- The 1.2 Å structure of a novel quorum-sensing protein, Bacillus subtilis LuxSJournal of Molecular Biology, 2001
- THINKING ABOUT BACTERIAL POPULATIONS AS MULTICELLULAR ORGANISMSAnnual Review of Microbiology, 1998