A Sycamore Cell Wall Polysaccharide and a Chemically Related Tomato Leaf Polysaccharide Possess Similar Proteinase Inhibitor-Inducing Activities

Abstract

A large pectic polysaccharide, called rhamnogalacturonan I, that is solubilized by a fungal endo-α-1,4-polygalacturonase from the purified walls of suspension-cultured sycamore cells possesses proteinase inhibitor-inducing activity similar to that of the proteinase inhibitor-inducing factor, a pectic-like oligosaccharide fraction isolated from tomato leaves. This suggests that the proteinase inhibitor-inducing activity resides in particular polysaccharide fragments which can be released when plant cell walls are exposed to appropriate enzyme degradation as a result of either wounding or pest attack.