Histidine inhibits oxidative stress‐ and TNF‐α‐induced interleukin‐8 secretion in intestinal epithelial cells
- 2 August 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (21), 4671-4677
- https://doi.org/10.1016/j.febslet.2005.07.038
Abstract
We investigated the effect of several amino acids on the secretion of such inflammatory cytokines as interleukin-8 (IL-8) induced by hydrogen peroxide or tumor necrosis factor-alpha (TNF-α) in intestinal epithelial-like Caco-2 and HT-29 cells. We found that histidine, one of the conditionally essential amino acids, significantly inhibited both hydrogen peroxide- and TNF-α-induced IL-8 secretion and mRNA expression in Caco-2 cells and HT-29 cells. These inhibitions were dose dependent and the inhibition rate of hydrogen peroxide-induced IL-8 secretion reached more than 50% at a concentration of 25mM, with over 95% inhibition at a concentration of 50mM. TNF-α increased the transcriptional activity of the IL-8 promoter which was significantly inhibited by treating Caco-2 cells with histidine. Histidine also abolished the NF-κB-dependent activation of the IL-8 promoter induced by TNF-α. These results indicate that histidine inhibited the hydrogen peroxide- and TNF-α-induced IL-8 secretion at the transcriptional level in intestinal epithelial cells, suggesting that histidine has the potential to attenuate intestinal inflammationKeywords
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