Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary
- 1 November 2009
- journal article
- research article
- Published by BMJ in International Journal of Gynecologic Cancer
- Vol. 19 (9), 1545-1549
- https://doi.org/10.1111/igc.0b013e3181a844c8
Abstract
Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.Abbreviations: CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1βKeywords
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