Antiviral Activity of Purified Human Breast Milk Mucin
- 14 March 2007
- journal article
- Published by S. Karger AG in Neonatology
- Vol. 92 (2), 96-104
- https://doi.org/10.1159/000100808
Abstract
Human breast milk is known to contain numerous biologically active components which protect breast fed infants against microbes, viruses, and toxins. The purpose of this study was to purify and characterize the breast milk mucin and determine its anti-poxvirus activity. In this study human milk mucin, free of contaminant protein and of sufficient quantity for further analysis, was isolated and purified by Sepharose CL-4B gel filtration and cesiumchloride density-gradient centrifugation. Based on the criteria of size and appearance of the bands and their electrophoretic mobility on sodium dodecyl sulfate polyacrylamide-gel electrophoresis, Western blotting together with the amino acid analysis, it is very likely that the human breast milk mucin is MUC1. It was shown that this breast milk mucin inhibits poxvirus activity by 100% using an inhibition assay with a viral concentration of 2.4 million plaque-forming units/ml. As the milk mucin seems to aggregate poxviruses prior to their entry into host cells, it is possible that this mucin may also inhibit other enveloped viruses such as HIV from entry into host cells.Keywords
This publication has 24 references indexed in Scilit:
- Lewis X component in human milk binds DC-SIGN and inhibits HIV-1 transfer to CD4+ T lymphocytesJCI Insight, 2005
- Anti‐HIV, Anti‐Poxvirus, and Anti‐SARS Activity of a Nontoxic, Acidic Plant Extract from the Trifollium Species Secomet‐V/anti‐Vac Suggests That It Contains a Novel Broad‐Spectrum AntiviralAnnals of the New York Academy of Sciences, 2005
- Lack of N1L Gene Expression Results in a Significant Decrease of Vaccinia Virus Replication in Mouse BrainAnnals of the New York Academy of Sciences, 2004
- The epithelial mucin, MUC1, of milk, mammary gland and other tissuesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1995
- Human milk mucin inhibits rotavirus replication and prevents experimental gastroenteritis.JCI Insight, 1992
- A Human Milk Factor Inhibits Binding of Human Immunodeficiency Virus to the CD4 ReceptorPediatric Research, 1992
- Human milk-fat globule membrane derived mucin is a disulfide-linked heteromerBiochemical and Biophysical Research Communications, 1989
- Amino acid analysis utilizing phenylisothiocyanate derivativesAnalytical Biochemistry, 1988
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- BIOCHEMICAL AND MORPHOLOGICAL COMPARISON OF PLASMA MEMBRANE AND MILK FAT GLOBULE MEMBRANE FROM BOVINE MAMMARY GLANDThe Journal of cell biology, 1970