Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2
Open Access
- 14 January 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (1), e16264
- https://doi.org/10.1371/journal.pone.0016264
Abstract
MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined. Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3′UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2. This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3′UTR region of EphA2 mRNA.This publication has 42 references indexed in Scilit:
- Restriction of Receptor Movement Alters Cellular Response: Physical Force Sensing by EphA2Science, 2010
- EphA2 overexpression correlates with poor prognosis in esophageal squamous cell carcinomaInternational Journal of Cancer, 2002
- Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations: Role in vasculogenic mimicryThe Prostate, 2002
- Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft.2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Molecular regulation of tumor cell vasculogenic mimicry by tyrosine phosphorylation: role of epithelial cell kinase (Eck/EphA2).2001
- EphA2 overexpression causes tumorigenesis of mammary epithelial cells.2001
- The ephrin-A1 ligand and its receptor, EphA2, are expressed during tumor neovascularizationOncogene, 2000
- Eph receptors and ephrin ligands: embryogenesis to tumorigenesisOncogene, 2000
- Overexpression of the EphA2 tyrosine kinase in prostate cancerThe Prostate, 1999