Modeling and analysis of Schistosoma Argonaute protein molecular spatial conformation
- 31 August 2011
- journal article
- Published by Medknow in Asian Pacific Journal of Tropical Biomedicine
- Vol. 1 (4), 275-278
- https://doi.org/10.1016/s2221-1691(11)60042-7
Abstract
To analyze the amino acid sequence composition, secondary structure, the spatial conformation of its domain and other characteristics of Argonaute protein. Bioinformatics tools and the internet server were used. Firstly, the amino acid sequence composition features of the Argonaute protein were analyzed, and the phylogenetic tree was constructed. Secondly, Argonaute protein's distribution of secondary structure and its physicochemical properties were predicted. Lastly, the protein functional expression form of the domain group was established through the Phyre-based analysis on the spatial conformation of Argonaute protein domains. 593 amino acids were encoded by Argonaute protein, the phylogenetic tree was constructed, and Argonaute protein's distribution of secondary structure and its physicochemical properties were obtained through analysis. In addition, the functional expression form which comprised the N-terminal PAZ domain and C-terminal Piwi domain for the Argonaute protein was obtained with Phyre. The information relationship between the structure and function of the Argonaute protein can be initially established with bioinformatics tools and the internet server, and this provides the theoretical basis for further clarifying the function of Schistosoma Argonaute protein.Keywords
This publication has 17 references indexed in Scilit:
- Recognition of 2′-O-Methylated 3′-End of piRNA by the PAZ Domain of a Piwi ProteinStructure, 2011
- Form, Function, and Regulation of ARGONAUTE ProteinsPlant Cell, 2010
- Sampling the Structure of the Noncanonical Lin-4:Lin-14 microRNA:mRNA Complex by Molecular Dynamics SimulationsThe Journal of Physical Chemistry B, 2010
- Sequencing of DC-SIGN promoter indicates an association between promoter variation and risk of nasopharyngeal carcinoma in cantoneseBMC Medical Genetics, 2010
- Mechanism of MicroRNA-Target Interaction: Molecular Dynamics Simulations and Thermodynamics AnalysisPLoS Computational Biology, 2010
- Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicumZeitschrift für Parasitenkunde, 2010
- Identification and characterization of microRNAs and endogenous siRNAs in Schistosoma japonicumBMC Genomics, 2010
- RNA interference in schistosomes: machinery and methodologyParasitology, 2009
- Redundant and Specific Roles of the ARGONAUTE Proteins AGO1 and ZLL in Development and Small RNA-Directed Gene SilencingPLoS Genetics, 2009
- Mapping the antigenic determinants and reducing the immunogenicity of trichosanthin by site-directed mutagenesisJournal of Biomedical Science, 2006