Effect of Exercise Training on Enos Expression, NO Production and Oxygen Metabolism in Human Placenta
Open Access
- 14 November 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (11), e80225
- https://doi.org/10.1371/journal.pone.0080225
Abstract
To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. The study included 20 nulliparous in gestational week 16–20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55–75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1–3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.Keywords
This publication has 33 references indexed in Scilit:
- Exercise for pregnant women for preventing gestational diabetes mellitusCochrane Database of Systematic Reviews, 2012
- Review: Reactive oxygen and nitrogen species and functional adaptation of the placentaPlacenta, 2010
- Clinical trial to assess the effect of physical exercise on endothelial function and insulin resistance in pregnant womenTrials, 2009
- Design of FitFor2 study: the effects of an exercise program on insulin sensitivity and plasma glucose levels in pregnant women at high risk for gestational diabetesBMC Pregnancy and Childbirth, 2009
- Regulation of mitochondrial uncoupling respiration during exercise in rat heart: Role of reactive oxygen species (ROS) and uncoupling protein 2Free Radical Biology & Medicine, 2008
- Flow-mediated dilatation of the brachial artery in pregnancyInternational Journal of Gynecology & Obstetrics, 2006
- Oxidative stress in the placentaHistochemistry and Cell Biology, 2004
- Enalapril Increases Mitochondrial Nitric Oxide Synthase Activity in Heart and LiverAntioxidants and Redox Signaling, 2003
- Committee opinion #267: exercise during pregnancy and the postpartum periodObstetrics & Gynecology, 2002
- Effect of Training on H2O2 Release by Mitochondria from Rat Skeletal MuscleArchives of Biochemistry and Biophysics, 1999