Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene
- 6 October 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (27), 6074-6078
- https://doi.org/10.1016/j.febslet.2005.09.073
Abstract
A cDNA encoding chalcone isomerase (CHI) was isolated from the petals of Nicotiana tabacum and the effect of its suppression on flavonoid biosynthesis was analyzed in transgenic tobacco plants. CHI-suppression by RNA interference (RNAi) showed reduced pigmentation and change of flavonoid components in flower petals. The plants also accumulated high levels of chalcone in pollen, showing a yellow coloration. Our results first demonstrated that suppression of CHI by genetic transformation is possible in higher plants. This suggests that CHI plays a major part in the cyclization reaction from chalcone to flavanone, and that spontaneous reactions are few, if any, in tobacco plants.Keywords
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