Bioactive neo-Clerodane Diterpenoids from the Whole Plants of Ajuga ciliata Bunge

Abstract

Ten new neo-clerodane diterpenes, ajugaciliatins A-J (1-5, 8-12), along with 17 known analogues (6, 7, 13-27) were isolated from the whole plants of Ajuga ciliata Bunge. Their structures were elucidated by spectroscopic data analysis (IR, ESIMS, HRESIMS, 1D and 2D NMR), and the configuration of 1 was confirmed by X-ray crystallography. All of the compounds were assessed for neuroprotective effects against MPP(+)-induced neuronal cell death in dopaminergic neuroblastoma SH-SY5Y cells. Compounds 2, 6, 7, 9, 10, 15-17, 19, and 20 exhibited moderate neuroprotective effects.