Interstrain transfer of the large pathogenicity island (PAPI-1) of Pseudomonas aeruginosa
- 26 December 2006
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (52), 19830-19835
- https://doi.org/10.1073/pnas.0606810104
Abstract
The large Pseudomonas aeruginosa pathogenicity island PAPI-1 of strain PA14 is a cluster of 108 genes that encode a number of virulence features. We demonstrate that, in a subpopulation of cells, PAPI-1 can exist in an extrachromosomal circular form after precise excision from its integration site within the 3' terminus of the tRNA(Lys) gene. Circular PAPI-1 can reintegrate into either of the two tRNA(Lys) genes, including the one that was used for integration of small pathogenicity island PAPI-2 in strain PA14. The excision requires PAPI-1-encoded integrase, a member of the tyrosine recombinase family. PAPI-1 Soj contains the conserved domains of proteins that are related to chromosome and plasmid partition. soj plays a role in maintaining PAPI-1 and mutations in soj result in the loss of PAPI-1 from P. aeruginosa. We further demonstrate that, during coculture, the PAPI-1-containing strains are able to transfer it into P. aeruginosa recipient strains that do not harbor this island naturally. After transfer, PAPI-1 integrates into either of the two tRNA(Lys) genes. PAPI-1 encompasses many features of mobile elements, including mobilization and maintenance modules. Together with the virulence determinants, PAPI-1 plays an important role in the evolution of P. aeruginosa, by expanding its natural habitat from soil and water to animal and human infections.Keywords
This publication has 38 references indexed in Scilit:
- Acquisition and Evolution of theexoULocus inPseudomonas aeruginosaJournal of Bacteriology, 2006
- Plasmid Segregation MechanismsAnnual Review of Genetics, 2005
- Effect of Metabolic Imbalance on Expression of Type III Secretion Genes in Pseudomonas aeruginosaInfection and Immunity, 2004
- Pathogenicity Islands in BacterialPathogenesisClinical Microbiology Reviews, 2004
- Gene Islands Integrated into tRNA Gly Genes Confer Genome Diversity on a Pseudomonas aeruginosa CloneJournal of Bacteriology, 2002
- Bacterial conjugation: a two‐step mechanism for DNA transportMolecular Microbiology, 2002
- Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variationNature, 2002
- Integrons: natural tools for bacterial genome evolutionCurrent Opinion in Microbiology, 2001
- Formation of Chromosomal Tandem Arrays of the SXT Element and R391, Two Conjugative Chromosomally Integrating Elements That Share an Attachment SiteJournal of Bacteriology, 2001
- Pathogenicity Islands and the Evolution of MicrobesAnnual Review of Microbiology, 2000