Functional analysis of colonic bacterial metabolism: relevant to health?
- 1 January 2012
- journal article
- review article
- Published by American Physiological Society in American Journal of Physiology-Gastrointestinal and Liver Physiology
- Vol. 302 (1), G1-G9
- https://doi.org/10.1152/ajpgi.00048.2011
Abstract
With the use of molecular techniques, numerous studies have evaluated the composition of the intestinal microbiota in health and disease. However, it is of major interest to supplement this with a functional analysis of the microbiota. In this review, the different approaches that have been used to characterize microbial metabolites, yielding information on the functional end products of microbial metabolism, have been summarized. To analyze colonic microbial metabolites, the most conventional way is by application of a hypothesis-driven targeted approach, through quantification of selected metabolites from carbohydrate (e.g., short-chain fatty acids) and protein fermentation (e.g., p-cresol, phenol, ammonia, or H2S), secondary bile acids, or colonic enzymes. The application of stable isotope-labeled substrates can provide an elegant solution to study these metabolic pathways in vivo. On the other hand, a top-down approach can be followed by applying metabolite fingerprinting techniques based on 1H-NMR or mass spectrometric analysis. Quantification of known metabolites and characterization of metabolite patterns in urine, breath, plasma, and fecal samples can reveal new pathways and give insight into physiological regulatory processes of the colonic microbiota. In addition, specific metabolic profiles can function as a diagnostic tool for the identification of several gastrointestinal diseases, such as ulcerative colitis and Crohn's disease. Nevertheless, future research will have to evaluate the relevance of associations between metabolites and different disease states.Keywords
This publication has 116 references indexed in Scilit:
- Sulfatases and a Radical S-Adenosyl-l-methionine (AdoMet) Enzyme Are Key for Mucosal Foraging and Fitness of the Prominent Human Gut Symbiont, Bacteroides thetaiotaomicronJournal of Biological Chemistry, 2011
- Emerging role of hydrogen sulfide in colonic physiology and pathophysiologyInflammatory Bowel Diseases, 2011
- High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissuesNature Protocols, 2010
- Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundant microbiota of young and elderly adultsEnvironmental Microbiology, 2009
- The gut microbiota shapes intestinal immune responses during health and diseaseNature Reviews Immunology, 2009
- Characterizing a model human gut microbiota composed of members of its two dominant bacterial phylaProceedings of the National Academy of Sciences of the United States of America, 2009
- Effects of the gut microbiota on host adiposity are modulated by the short-chain fatty-acid binding G protein-coupled receptor, Gpr41Proceedings of the National Academy of Sciences, 2008
- Symbiotic gut microbes modulate human metabolic phenotypesProceedings of the National Academy of Sciences, 2008
- Mass spectrometry‐based metabolomicsMass Spectrometry Reviews, 2006
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999