Microfluidic sorting in an optical lattice
Top Cited Papers
- 27 November 2003
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 426 (6965), 421-424
- https://doi.org/10.1038/nature02144
Abstract
The response of a microscopic dielectric object to an applied light field can profoundly affect its kinetic motion1. A classic example of this is an optical trap, which can hold a particle in a tightly focused light beam2. Optical fields can also be used to arrange, guide or deflect particles in appropriate light-field geometries3,4. Here we demonstrate an optical sorter for microscopic particles that exploits the interaction of particles—biological or otherwise—with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice. The strength of this interaction with the lattice sites depends on the optical polarizability of the particles, giving tunable selection criteria. We demonstrate both sorting by size (of protein microcapsule drug delivery agents) and sorting by refractive index (of other colloidal particle streams). The sorting efficiency of this method approaches 100%, with values of 96% or more observed even for concentrated solutions with throughputs exceeding those reported for fluorescence-activated cell sorting5. This powerful, non-invasive technique is suited to sorting and fractionation within integrated (‘lab-on-a-chip’) microfluidic systems, and can be applied in colloidal, molecular and biological research.This publication has 18 references indexed in Scilit:
- Brownian Particle in an Optical Potential of the Washboard TypePhysical Review Letters, 2003
- Electrodeless Dielectrophoresis of Single- and Double-Stranded DNABiophysical Journal, 2002
- Kinetically Locked-In Colloidal Transport in an Array of Optical TweezersPhysical Review Letters, 2002
- Brownian Motion of DNA Confined Within a Two-Dimensional ArrayScience, 2002
- Separation of Long DNA Molecules in a Microfabricated Entropic Trap ArrayScience, 2000
- A microfabricated fluorescence-activated cell sorterNature Biotechnology, 1999
- Microfabricated Sieve for the Continuous Sorting of MacromoleculesPhysical Review Letters, 1998
- Lateral Separation of Macromolecules and Polyelectrolytes in Microlithographic ArraysPhysical Review Letters, 1998
- Optical Matter: Crystallization and Binding in Intense Optical FieldsScience, 1990
- Observation of a single-beam gradient force optical trap for dielectric particlesOptics Letters, 1986