LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues
Open Access
- 19 January 2012
- journal article
- Published by Springer Science and Business Media LLC in BMC Research Notes
- Vol. 5 (1), 45
- https://doi.org/10.1186/1756-0500-5-45
Abstract
Background Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium. Findings We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids. Conclusions The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.Keywords
This publication has 15 references indexed in Scilit:
- Isolation of high-quality RNA from various tissues of Jatropha curcas for downstream applicationsAnalytical Biochemistry, 2011
- An RNA isolation system for plant tissues rich in secondary metabolitesBMC Research Notes, 2011
- Isolation of good quality RNA from a medicinal plant seabuckthorn, rich in secondary metabolitesPlant Physiology and Biochemistry, 2009
- Phenol burnBurns, 2006
- Rose ScentTHE PLANT CELL ONLINE, 2002
- Isolation of nucleic acids from plants by differential solvent precipitationAnalytical Biochemistry, 1991
- Improved method for the isolation of RNA from plant tissuesAnalytical Biochemistry, 1987
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987
- Cardiac Dysrhythmias Associated with Chemical Peeling with PhenolAnesthesiology, 1985