Adenovirus-Mediated Expression of Interferon-α Delays Viral Replication and Reduces Disease Signs in Swine Challenged with Porcine Reproductive and Respiratory Syndrome Virus
- 1 June 2009
- journal article
- research article
- Published by Mary Ann Liebert Inc in Viral Immunology
- Vol. 22 (3), 173-180
- https://doi.org/10.1089/vim.2008.0075
Abstract
In this study, pigs were injected with a nonreplicating human adenovirus type 5 vector expressing porcine interferon-α (Ad5-pIFN-α) and then challenged with porcine reproductive and respiratory syndrome virus (PRRSV) to determine whether the presence of increased levels of IFN-α would decrease viral replication and/or disease. Groups of 10 pigs each were inoculated with Ad5-pIFN-α and not challenged, Ad5-pIFN-α and challenged with PRRSV 1 d later, or inoculated with a control adenovirus that does not express IFN-α (Ad5-null) and challenged 1 d later with PRRSV. IFN-α levels in all pigs inoculated with the Ad5-pIFN-α were elevated the day of challenge (1 d after inoculation), but were undetectable by 3 d after inoculation in the pigs that were not challenged with PRRSV. Pigs inoculated with Ad5-pIFN-α and challenged with PRRSV had lower febrile responses, a decreased percentage of lung involvement at 10 d post-infection, delayed viremia and antibody response, and higher serum IFN-α levels as a result of PRRSV infection, compared to pigs inoculated with Ad5-null and challenged with PRRSV. These results indicate that IFN-α can have protective effects if present during the time of infection with PRRSV.Keywords
This publication has 35 references indexed in Scilit:
- Differences in Clinical Disease and Immune Response of Pigs Challenged with a High-Dose versus Low-Dose Inoculum of Porcine Reproductive and Respiratory Syndrome VirusViral Immunology, 2008
- Recombinant swine beta interferon protects swine alveolar macrophages and MARC-145 cells from infection with Porcine reproductive and respiratory syndrome virusJournal of General Virology, 2007
- Differential type I interferon activation and susceptibility of dendritic cell populations to porcine arterivirusImmunology, 2006
- Immune responses and protection by vaccine and various vaccine adjuvant candidates to virulent porcine reproductive and respiratory syndrome virusVeterinary Immunology and Immunopathology, 2006
- Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virusVeterinary Immunology and Immunopathology, 2004
- Porcine reproductive and respiratory syndrome virus field isolates differ in in vitro interferon phenotypesVeterinary Immunology and Immunopathology, 2004
- Differential production of proinflammatory cytokines in the pig lung during different respiratory virus infections: correlations with pathogenicityResearch in Veterinary Science, 1999
- The neglected role of type I interferon in the T-cell response: implications for its clinical useImmunology Today, 1996
- A sensitive immunoassay for porcine interferon-αVeterinary Immunology and Immunopathology, 1992
- Appearance of interferon-α in serum and signs of reduced immune function in pigs after transport and installation in a fattening farmVeterinary Immunology and Immunopathology, 1989