Epidermal patterning genes are active during embryogenesis in Arabidopsis
Open Access
- 1 July 2003
- journal article
- Published by The Company of Biologists in Development
- Vol. 130 (13), 2893-2901
- https://doi.org/10.1242/dev.00493
Abstract
Epidermal cells in the root of Arabidopsis seedling differentiate either as hair or non-hair cells, while in the hypocotyl they become either stomatal or elongated cells. WEREWOLF (WER) and GLABRA2 (GL2) are positive regulators of non-hair and elongated cell development. CAPRICE (CPC) is a positive regulator of hair cell development in the root. We show that WER, GL2 and CPC are expressed and active during the stages of embryogenesis when the pattern of cells in the epidermis of the root-hypocotyl axis forms. GL2 is first expressed in the future epidermis in the heart stage embryo and its expression is progressively restricted to those cells that will acquire a non-hair identity in the transition between torpedo and mature stage. The expression of GL2 at the heart stage requires WER function. WER and CPC are transiently expressed throughout the root epidermal layer in the torpedo stage embryo when the cell-specific pattern of GL2 expression is being established in the epidermis. We also show that WER positively regulates CPC transcription and GL2 negatively regulates WER transcription in the mature embryo. We propose that the restriction of GL2 to the future non-hair cells in the root epidermis can be correlated with the activities of WER and CPC during torpedo stage. In the embryonic hypocotyl we show that WER controls GL2 expression. We also provide evidence indicating that CPC may also regulate GL2 expression in the hypocotyl.Keywords
This publication has 16 references indexed in Scilit:
- Role of a positive regulator of root hair development,CAPRICE,inArabidopsisroot epidermal cell differentiationDevelopment, 2002
- TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in ArabidopsisThe EMBO Journal, 2002
- Cell Pattern in the Arabidopsis Root Epidermis Determined by Lateral Inhibition with FeedbackPlant Cell, 2002
- WEREWOLF, a MYB-Related Protein in Arabidopsis, Is a Position-Dependent Regulator of Epidermal Cell PatterningCell, 1999
- The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat ProteinPlant Cell, 1999
- The development and evolution of bristle patterns in DipteraDevelopment, 1999
- A Common Position-Dependent Mechanism Controls Cell-Type Patterning and GLABRA2 Regulation in the Root and Hypocotyl Epidermis of Arabidopsis1Plant Physiology, 1998
- Stomata Patterning on the Hypocotyl ofArabidopsis thalianaIs Controlled by Genes Involved in the Control of Root Epidermis PatterningDevelopmental Biology, 1998
- The Arabidopsis Athb‐10 (GLABRA2) is an HD‐Zip protein required for regulation of root hair developmentThe Plant Journal, 1996
- The TTG Gene Is Required to Specify Epidermal Cell Fate and Cell Patterning in the Arabidopsis RootDevelopmental Biology, 1994