Glutamate‐stimulated ATP release from spinal cord astrocytes is potentiated by substance P
- 8 August 2006
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 99 (3), 924-936
- https://doi.org/10.1111/j.1471-4159.2006.04133.x
Abstract
ATP has recently emerged as a key molecule mediating pathological pain. The aim of this study was to examine whether spinal cord astrocytes could be a source of ATP in response to the nociceptive neurotransmitters glutamate and substance P. Glutamate stimulated ATP release from these astrocytes and this release was greatly potentiated by substance P, even though substance P alone did not elicit ATP release. Substance P also potentiated glutamate-induced inward currents, but did not cause such currents alone. When glutamate was applied alone it acted exclusively through alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionate receptors to stimulate Ca(2+) influx-dependent ATP release. However, when substance P was co-applied with glutamate, ATP release could be elicited by activation of NMDA and metabotropic glutamate receptors. Activation of neurokinin receptor subtypes, protein kinase C and phospholipases A(2), C and D were needed for substance P to bring about its effects. These results suggest that astrocytes may be a major source of ATP in the spinal cord on activation of nerve fibres that release substance P and glutamate.Keywords
This publication has 56 references indexed in Scilit:
- Secretion of ATP from Schwann cells in response to uridine triphosphateEuropean Journal of Neuroscience, 2005
- Low-mode docking search in iGluR homology models implicates three residues in the control of ligand selectivityJournal of Molecular Recognition, 2004
- Expression of P2X7 receptor immunoreactivity in distinct subsets of synaptic terminals in the ventral horn of rat lumbar spinal cordBrain Research, 2004
- Mechanisms of secretion of ATP from cortical astrocytes triggered by uridine triphosphateNeuroReport, 2003
- Functional Coupling of the NK1 Tachykinin Receptor to Phospholipase D in Chinese Hamster Ovary Cells and Astrocytoma CellsJournal of Neurochemistry, 2002
- Evidence that the substance P‐induced enhancement of pacemaking in lymphatics of the guinea‐pig mesentery occurs through endothelial release of thromboxane A2British Journal of Pharmacology, 1997
- Spinal cord astrocytes in vitro: Phenotypic diversity and sodium channel immunoreactivityGlia, 1993
- Intrathecal co-administration of substance P and NMDA augments nociceptive responses in the formalin testPain, 1992
- Ventral medulla stimulation increases blood pressure and spinal cord amino acid releaseNeuroReport, 1992
- Localization of the thiorphan-sensitive endopeptidase, termed enkephalinase A, on glial cellsFEBS Letters, 1983